Each group needs:

• 6 2-liter soda bottles
• 3 2-liter bottle caps
• 3 500-ml plastic water bottles with caps
• 1.5 meters (5 ft) thin tubing, such as fish tank aeration tubing, which is reusable and can be used for a number of other activities; such as aquarium airline tubing with 0.165-inch inner diameter and 0.225-inch outer diameter, available in various lengths from https://www.petmountain.com/product/lees-airline-tubing-for-aquariums
• 1.5 liters cow manure, or sediment from the bottom of a pond, swamp or other consistently wet area
• 1 hot dog
• lab gloves, one pair per student
• safety goggles, one per student
• lab coat, one per student
• sharpie or similar marker for labeling plastic bottles
• graduated cylinder
• Anaerobic Digestion Data Sheet, one per group
• Anaerobic Digestion Worksheet, one per student

To share with the entire class:

• hot glue gun and glue sticks
• drill and a drill bit that is slightly larger than the aeration tubing diameter; alternatively, use a knife to cut holes in bottle caps
• measuring cup
• bucket of tap water that has been left sitting out at room temperature for 24 hours (to help the chlorine dissipate); need at least 1.5 liters per group
• scissors, for cutting plastic bottles and tubing
• knife and cutting board, to cut hot dogs
• graph paper, colored pencils/markers, for plotting graphs