Materials List:
Cooked, Salted, or Lysed—the Survivor:
Investigative Options that Select for Polyvalency or Polyresistance
https://www.teachengineering.org/activities/view/rice-2789-polyvalency-polyresistance-organisms-bacteriophage-activity
Investigative Options that Select for Polyvalency or Polyresistance
Materials for Option 1
- personal protection equipment (PPE)
- autoclave
- culture chambers
- laminar flow benches (or a comparable sterile workbench)
- storage under refrigeration
- micropipettes
- Parafilm M®
- petri dishes
- live bacterial hosts are available from Carolina.com, such as Escherichia coli and Pseudomonas putida (2-4 bacterial hosts are recommended)
- Carolina.com has phage culture kits
Materials for Option 2
Each group needs:
- 20 mung beans seeds / group
- NaCl
- distilled water
- petri dishes
- cotton gauze/sterile paper towels
- 500 mL - 1 L beakers
- stirring rod
- hot plate
- strainer with handle
- fine-mesh bag
- twine
- access to sink with cold water
- small stone or other weight
- thermometer
- forceps or tweezers
- timer